Farhan Syafiq

3 Questions 3 Answers 0 Followers

Questions related from Farhan Syafiq

Calculation for miRNA expression level

Hi, I have treated breast cancer cell line (MCF7) with miRNA mimic. I want to calculate the fold change of miRNA expression between untreated and treated cells. However, the Ct value of miRNA in...

03 January 2017 5,341 3 View

Any advice on MTT reagent preparation (M6494 - Life Technologies)?

Hi, I just got MTT reagent (1g) from Life Technologies, but I don't know how to prepared it from. I tried to find in the website but got nothing. What filter size can I use? What concentration...

08 December 2015 8,711 6 View

How do I improve the purity of exosomes?

I successfully isolated exosomes from endometrial THESC but the RNA yield is low (1.29)? Any suggestion how to improve it ?

08 September 2015 4,152 4 View