Fareed Sairi

5 Questions 20 Answers 0 Followers

Questions related from Fareed Sairi

Why did my synthesised peptide from DRAMP have no antimicrobial activity?

Hello everyone, I'm working with predicted antimicrobial peptide using machine learning. Recently, we use an antimicrobial peptide from DRAMP which had been verified to have antimicrobial...

03 January 2024 835 10 View

What is the best bioinformatic tool to predict the interaction of peptide with bacterial cell wall membrane?

Hello everyone, How are you guys doing? I'm currently working with antimicrobial peptide (AMP) project and one of our objectives is to predict the interaction of peptide against bacterial cell...

02 February 2021 1,659 3 View

Has anyone tried competitive FISH using single mRNA oligonucleotides probe on mRNA sequence with isoforms?

I'm interested to know if anyone had tried competitive FISH on mRNA sequence of 60% similar isoforms. What if these isoform did not have decent nucleotides gaps to provide specific probes? Is it...

10 September 2013 2,109 2 View

How to prevent clumps/aggregation of cells during permeabilization for FISH experiment?

I'm currently having a problem with clumps of cells after cell permeabilization treatment using 70% ethanol. I want to permeabilize suspension cells and treat with FISH probes. After that, the...

17 June 2013 8,473 7 View

Is there a negative effect of air-drying on cell suspension prior to fix and permeability for FISH and Immunocytochemistry?

I have a suspension cell, which cannot adhere to the surface. I had to use air-drying methods to perform my immunocytochemistry, with average result. I still need some more optimization. I'm also...

12 November 2012 2,928 10 View