How do you improve the dynamic range of a fluorescence polarization assay using RNA and a fluorescent-peptide?

I am trying to replicate the FP assay from the following publication and am wondering why the change in mp value between RNA(70-nt long, 23 KDa) and the fluorescent-peptide (12-mer, 2 kDa) is very...

19 September 2014 5,502 2 View