How can I improve my lentivirus titer?

I'm using ~P5 fast-dividing (time to double ~ 1 day) HEK cells grown to about 90% confluence in a T75 flask. Cells were kept in DMEM (+high glucose, +glutamine) + 10% HI-FBS + Anti-Anti until the...

26 June 2015 1,842 9 View