Farah Alhaman

2 Questions 1 Answers 0 Followers

Questions related from Farah Alhaman

A Question About a Difficult Cloning of a Plasmid for Downstream Transfection into Drosophila S2 Cells?

Hi everyone! I have been trying to do a cloning of a plasmid that should , in theory, have been straightforward. I took a PMT_CID_V5_6His_Hygro plasmid from my labs' plasmid stocks and cut the V5...

last month 9,657 1 View

Why may I get a Ct value that is higher for one of my treated samples than my control ( zero hour in a time course study) in RT qPCR??

I'm conducting a time course study on gene expression using RT-q PCR for samples treated with 4 conditions: vehicle, RA agonist, Calcitriol agonist or a combination of both agonists over 6 hours....

27 January 2024 7,955 1 View