Why may I get a Ct value that is higher for one of my treated samples than my control ( zero hour in a time course study) in RT qPCR??

I'm conducting a time course study on gene expression using RT-q PCR for samples treated with 4 conditions: vehicle, RA agonist, Calcitriol agonist or a combination of both agonists over 6 hours....

27 January 2024 7,901 1 View