Trouble detecting high molecular weight GFP-tagged AnkG and NF186 by Western blot — any advice?

I'm working with two high molecular weight proteins: GFP-tagged Ankyrin-G (AnkG, 270 kDa + GFP = ~297 kDa) GFP-tagged NF186 (213 kDa total) Both are expressed in mammalian cells via transfection....

19 May 2025 2,370 2 View