Faizan Malik

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Questions related from Faizan Malik

Embryonic brain free floating 50um sections - immunofluorescence antibody penetration iba1/mounting question?

Hello all, I am having trouble with antibody penetration of Iba1 staining in my tissue, i can only seem to get about a 30-35 z-stack. Additionally I have a really hard time mounting this tissue,...

27 February 2020 8,258 5 View

How to optimize RNA yield for low number of FAC sorted cells?

Hi Everyone, I have been working with a FAC sorted population of embryonic brain cells. I typically get between 50-90% viability following the sort but only 12 000 - 20 000 cells. I have been...

24 May 2019 4,288 5 View