Do I need isolate CD4 positive cells by MACS first for IL-22 intracellular staining in mouse MLN lymphocytes?

Recently I'm working on detecting IL22-producing CD4 positive cells in mouse MLN by flow cytometry. However, after using PMA/ionomycin plus GolgiPlug neither 4 hrs or 6 hrs culture, IL-22 positive...

02 September 2015 834 3 View