Faezeh Ahrari

4 Questions 9 Answers 0 Followers

Questions related from Faezeh Ahrari

Why is my protein in the flow-through and wash but not in the elute?

I have expressed the enzyme salicylic acid decarboxylase and I want to purify it using FPLC but all my protein comes out in the flow through and wash buffers, meaning there is no binding between...

05 May 2025 7,634 7 View

How can I cleavage the disulfide bond and create two SH groups?

I have a disulfide bond and have tried several methods to break it, but none have been successful. Could you recommend an effective method for this?

01 January 2025 5,300 6 View

How to apply the method used for an HPLC column to an HPLC column with other specifications?

I want to apply the method used for the extraction and separation of an amide compound by HPLC analysis with a column (9.4 x 150 mm) for a column with Specifications (4.6 x 250 mm), What should I...

08 November 2024 5,526 4 View

How can I calculate Gibbs free energy for ligand-protein in Schrödinger?

I have done the docking process using Schrödinger for several ligand-proteins, how to calculate the value of ΔG for each of the ligand-proteins? regards

20 February 2024 5,910 3 View