How frequent and complete is deamidation of asparagine and glutamine residues in proteins fragmented with CNBr in 70% formic acid?

I am analyzing ESI/MS and MALDI/TOF MS spectra of a small protein subjected to CNBr digestion in 70% formic acid at room temp overnight and then digested with Asp-N proteinase. There appear to be...

11 January 2025 5,809 0 View