Fabiana Kühne

2 Questions 9 Answers 0 Followers

Questions related from Fabiana Kühne

How do you amplify a full length high CG cDNA for cloning using tagged primers?

I need to clone a full cDNA for protein expression (1782bp) and I have primers tagged with enzyme binding sites. I've tried to amplify the cDNA using different Tms and 5% DMSO in PureTaq...

06 June 2013 1,438 7 View

How many cDNA are you using for Real Time PCR in relative quantification and TaqMan?

Some people used to quantificate cDNA using spectrophotometer, another one prefers to use a volume of transcription without quantification... What do you do?

05 May 2013 2,271 11 View