I am using NEB 10-beta competent E.coli cells. Transformation is successful but colonies dont grow in LB. Do you have any advise?

I am using NEB PCR cloning Kit. There is pMiniT vector inside kit. I transformed ligation bwt my PCR product and pMiniT vector to NEB 10-beta competent E.coli cells. I used SOC growth medium for...

22 February 2016 2,713 7 View