Ewan St. John Smith

4 Questions 7 Answers 0 Followers

Questions related from Ewan St. John Smith

Bone disc recommendations for osteoclast work?

Hello! Does anyone have any recommendations for bone discs for looking at osteoclast activity? We're considering these (https://www.idsplc.com/products/bone-slices-2/), but they are currently...

27 October 2023 298 0 View

What's the best method for shipping dorsal root ganglion neurones for culturing?

I'm looking to receive DRG neurones from a collaborator that need to be shipped overnight for culturing at our end for electrophysiology analysis. Has anyone experience with the best method to...

26 February 2020 8,652 5 View

How best to grow active osteoclasts?

Hi all, I would like to set up a system for culturing active osteoclasts and I was wondering what people would recommend for a substrate? I know that dentin and bone can be used, but I was...

29 March 2016 4,606 3 View

How to decrease PFA-induced background fluroescence?

We are using Lumafluor red retrobeads to label DRG neurones, which works brilliantly when recording from neurones in culture. However, for immunohistochemical studies we observe high background...

25 November 2014 7,869 10 View