Why in my casein zymography, the gel was stained unevenly and patchy?

I have tried to make new solutions required for gel casting (30% acrylamide/bis, Tris-HCl pH 8.8 and 6.8, casein 0.5%), sample buffer, the staining solution and destaining solution, because the...

05 May 2019 7,550 2 View