What should I do if HPLC pump doesn’t deliver the eluent continuously and the pressure is fluctuating?

I use a reverse phase Waters HPLC. When I used the pump C last week the pressure suddenly fell down from 300 PSI to 0 (with 100% methanol, 0.2 ml/min) for a moment and than went back to 300. I...

19 May 2016 9,680 4 View