Eva Maria Deiner

2 Questions 6 Answers 0 Followers

Questions related from Eva Maria Deiner

Can someone advise an alternative to the "super Pap Pen"?

Since I have to stain cryosections with several antibodies on one slide for IF or PLA, I use the Super Pap Pen to encircle the sections, but obviously this pen cannot stand the O.C.T. and always...

08 August 2013 7,315 5 View

How can I stain primary B cells (mouse) with an antibody against mouse without having all their antibodies detected by the anti mouse sec. ab in IF?

I already tried blocking, which was fine for lines, but the B cells present so many antibodies that I cannot cover. The secondary antibody control still shows massive cytoplasmic/membrane...

02 February 2013 2,903 12 View