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Hi, I am having so so so much trouble getting LC3 on WB. Here is what I have been doing: Conditions: HeLa cells, AA starvation, EBSS, Rapamycin, Torin 1. They are all with/without NH4Cl+Leupeptin...
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Hi fellow researchers, I am having trouble performing live cell imaging with lysotracker blue using LSM780. Goal of my experiment is to track lysosome upon treatment, and I am using lysotracker...
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