Eunice Wong

2 Questions 6 Answers 0 Followers

Questions related from Eunice Wong

How to buffer extracellular calcium during bath-loading of chemical calcium indicator?

I am trying to calcium-image marine invertebrate larvae, by incubating larvae in FSW in AM-ester dye (Fluo-4 AM). I am wondering if dye saturation is the reason I'm not observing any signal...

25 January 2019 3,283 2 View

Next-Seq sequences mapping poorly to ref genome?

Hi We've just started using the Illumina Next-Seq platform and haven't been getting good results sequencing our CEL-Seq2 library (75cycle, R1=15bp, R2=77bp), as opposed to what we've been getting...

24 August 2018 7,605 7 View