Eunhye Kim

2 Questions 1 Answers 0 Followers

Questions related from Eunhye Kim

Has anyone experienced too much non-specific binding/background staining in IF imaging of liver tissue?

I stained various markers (CD3, CD8, CLEC4F, etc) in liver tissue containing metastatic tumor nodules for immunofluorescence imaging, but when I tried to take a picture showing tumor region and...

24 January 2024 5,173 1 View

What is a correct/best way to isolate Kupffer cell for flow cytometry analysis?

I have been trying to analyze Kupffer cell from C57BL/6 mouse liver by flow cytometry following standard protocols: 1. Liver harvest (I tried both before and after perfusion) 2. Liver chopping...

27 October 2023 3,215 1 View