Ethan Matthews

5 Questions 5 Answers 0 Followers

Questions related from Ethan Matthews

Why does the absorbance at 340nm decrease when it should increase for my enzyme activity assay?

Hi, I’m measuring the production of NADH so the A340 should be increasing but instead its decreasing. Its even more strange because I blank and autozero before I add the NAD. My enzyme is...

07 February 2023 8,923 3 View

Which does E. coli prefer more as a carbon source, sorbitol or glycerol?

The reason why I ask is because I want to add sorbitol to be terrific broth with glycerol but I'm worried it will preferentially metabolize the sorbitol. I want to add sorbitol because I've read...

05 January 2023 6,974 3 View

Why is my Strep-tactin lysate clogging the column?

Hello everyone, To increase the binding of my StrepII tagged protein to the Strep-Tactin®XT 4Flow high capacity resin, I have been incubating the resin with my lysate before adding to a column. I...

12 October 2022 4,935 6 View

Why is my Strep-tactin lysate clogging the column?

Hello everyone, To increase the binding of my StrepII tagged protein to the Strep-Tactin®XT 4Flow high capacity resin, I have been incubating the resin with my lysate before adding to a column....

21 April 2022 2,931 4 View

Does increasing shaker speed result in inclusion bodies?

Hello everyone, I have a protocol to express a enzyme (human 17B-HSD1), but it doesn't list the shaker speed, just the temp (18C for 12-18 hours). I obviously don't want inclusion bodies but I...

22 December 2021 7,826 3 View