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Questions related from Eswara Murali Satyavarapu
I tried to extract enzyme digested plasmid of size 6kb using crystal violet gel, but I only got 10% recovery. I used the protocol (see attachment). For purification I used the QIAgen gel...
04 April 2014 6,138 0 View
I had set the PCR reaction with LA taq and total volume is 25 microliters, it worked well. Same reaction, everything doubled and the final rxn volume is 50 microliters, it did not work. What is...
09 September 2013 4,761 11 View
