Esther Mezhibovsky

3 Questions 4 Answers 0 Followers

Questions related from Esther Mezhibovsky

Is there a way to control food intake in mice prior to tissue/sample collection?

I am looking to measure short chain fatty acids, carbohydrates, triglycerides, and cholesterol in the intestinal contents, intestinal tissue, portal vein, liver and cardiac serum. I will also be...

25 March 2020 7,322 3 View

How do you measure the pH of cecal content?

I have attempted vortexing in 200uL of 0.9% saline, but I found that the saline pH varied unless it was being spun. Because of the small volume of cecum collected I cannot dilute to a large enough...

15 July 2018 7,666 1 View

How do you choose FITC-Dextran size for evaluating intestinal permeability?

I am looking to test paracellular intestinal permeability in mice on different diets. I am reading up on FITC-Dextran assays and am wondering what size molecules I should purchase.

27 April 2018 2,192 3 View