What antibiotic concentration should I use for transient expression in CHO K1? Or is it not necessary to use antibiotics for selection?

I'm performing transient expression experiments with CHO K1 (suspension adapted). I'm using a pcDNA3.1 vector and PEI and Geneticin for selection. In the protocol I use (Geisse, 2006) nothing is...

22 January 2016 1,153 4 View