Estefania Elorriaga

3 Questions 4 Answers 0 Followers

Questions related from Estefania Elorriaga

Should I rehydrate frozen pollen before extracting RNA? or before germinating it in-vitro?

Hello. I will be extracting RNA from frozen eucalypt pollen soon. It has been in the -80 freezer for over a year. Should I rehydrate it before I extract RNA? I will also be germinating some...

15 May 2017 309 3 View

What is the recommended coverage for de novo genome sequencing?

Hello, We plan on resequencing a hybrid genotype of poplar (genome size~ 430Mb) and sequencing de novo a hybrid genotype of eucalyptus (genome size~ 580Mb). We would like to do as many lines as...

09 February 2017 357 3 View

I was wondering if anyone knew about studies involving CRISPRi for generating pathogen-resistant crops?

I was wondering if anyone has tried (and been successful) at generating pathogen-resistant plants using CRISPR interference (CRISPRi). The pathogen in question is an ascomycete fungus. Does...

10 February 2015 7,142 3 View