Esben Schmidt

3 Questions 1 Answers 0 Followers

Questions related from Esben Schmidt

How do you harvest mature human monocyte derived dendritic cells?

Human monocyte derived dendritic cells, through in vitro GM-CSF + IL-4 differentiation, are loosely- and non-adherent at their immature state. In my hands, maturation of such dendritic cells (LPS,...

23 November 2022 7,953 2 View

What's the optimal cell seeding density in Caco-2 transwell culture? And why?

What are the consequenses of too low/high seeding densities of Caco-2 cells, or other intestinal epithelial cell lines, in transwell culture systems? Or does cell concentration at seeding not...

18 August 2017 1,899 1 View

Does hematoxylin stain bacterial DNA (both intracellular DNA and secreted extracellular DNA)?

I want to visualize bacterial DNA with a stain that can be seen in bright light (no fluorescence etc.). What's best? Hematoxylin?

08 December 2015 3,612 1 View