Erin Unruh

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Questions related from Erin Unruh

Citrate Synthase reactivation assay--Acetyl CoA is laced with CoA--what to do?

Hi everyone, I am attempting a citrate synthase reactivation assay modeled after several previous assays I have found in literature. In short, I am using oxaloacetate and Acetyl-CoA to determine...

29 November 2022 6,061 0 View

Purifying protein from pellet, purifies in 4M urea, but precipitates during dialysis??

Hi all, I am purifying a human construct from Origami cells. Induce at 0.7 OD with 0.5mM IPTG at 16C for 16hours (have also tried 18C for 16 and 18 hours). My protein falls to the pellet....

03 September 2020 8,400 4 View