3 Questions 4 Answers 0 Followers
Questions related from Eriks Smagris
Hi. I have faced a problem I never seen before. I have Origene pCMV-XL6 Entry plasmid without any ORF - just empty plasmid. It has Kanamycin resistance so I transform plasmid in DH5a E. coli...
18 March 2020 3,426 4 View
Hi, Does anyone has used AAV (not adenovirus) for rats? I would like to overexpress and knock down several genes in rat liver. Any suggestions? Strain of AAV and dosage? Thanks
22 November 2017 5,630 2 View
Hi, I would like to make protein stably expressed cell line from HepG2 cells. Antibiotic selection working fine. Whenever I tried to select a single cell for a colony (1 cell per 200ul media) and...
08 June 2016 3,408 8 View
