Has anyone that has used IBI KleenGreen Master mix for qPCR experienced high readings for Beta Actin. If so how were you able to fix this problem?

We've tried to increase the concentration of cDNA and making sure that the correct ROX concentration was added. But we keep getting the same results, Beta Actin that is reading at 24 and above....

19 May 2016 4,620 4 View