Erick Awuoche

2 Questions 3 Answers 0 Followers

Questions related from Erick Awuoche

Any advise on the best cell lysis buffer or what to do to use Talon cobalt resin?

I am having problem purifying his-tagged protein using Talon cobalt resin. I can purify same protein Ni-his but I get some none specific binding proteins in the elution fractions. Cells were lysed...

01 April 2025 5,031 5 View

Problem with Mirdeep2 and Randfold output?

Hello team, I am using Mirdeep2 to identify miRNA in my organism (none model organism) which has no previously identified micro_RNAs. My organism is genetically close to Drosophila melanogaster....

15 April 2024 9,431 0 View