Erica Nathan

3 Questions 9 Answers 0 Followers

Questions related from Erica Nathan

How to ensure you get the entire pancreas from a mouse?

I have been doing invivo experiments with nude mice, wherein I have to isolate certain organs. During isolation, we are not sure if we are getting the entire pancreas as it is crucial for our...

28 October 2014 783 12 View

What is the effect of Arginine on siRNA binding?

My peptide has a siRNA binding domain. After synthesis and purification of peptide through Ni-NTA chromatography under denaturing conditions my protein would aggregate, so I added 10 mM arginine...

24 July 2014 560 0 View

How can I prevent protein aggregation and loss during refolding and storage?

I have a recombinant protein, which I purify using Ni-NTA chromatography in denaturing conditions (8M Urea). It comes in high concentrations, but upon refolding there is aggregation and loss and...

03 July 2014 8,190 9 View