Erica Nathan

2 Questions 6 Answers 0 Followers

Questions related from Erica Nathan

What is the effect of Arginine on siRNA binding?

My peptide has a siRNA binding domain. After synthesis and purification of peptide through Ni-NTA chromatography under denaturing conditions my protein would aggregate, so I added 10 mM arginine...

24 July 2014 474 0 View

How can I prevent protein aggregation and loss during refolding and storage?

I have a recombinant protein, which I purify using Ni-NTA chromatography in denaturing conditions (8M Urea). It comes in high concentrations, but upon refolding there is aggregation and loss and...

03 July 2014 8,148 9 View