Eric Owusu Obeng

3 Questions 7 Answers 0 Followers

Questions related from Eric Owusu Obeng

How do you quantify transwell invasion assays after staining with crystal violet? Anything else apart from counting cells manually?

I am trying to compare the invasiveness among to cell types I am working with via a transwell invasion assay. I am used to counting manually under the microscope, something which I think can be...

11 November 2019 7,768 3 View

Endogenous gene is expressing at a higher ct than target genes. What could be the cause? ps. RNA obtained from FFPE human brain tissue?

Dear all, my endogenous gene (b-actin to be precise) is expressing at a higher CT (30-32) than my target gene (ct= 28-29). RNA are from FFPE human brain tissues (GBM origin) and quality checked...

11 November 2019 585 3 View

Anyone tried extracting RNA from only Formalin fixed tissues (NB. only formalin without paraffin). If yes, do you get quality RNA?

I am aware of various commercial kits for extracting RNA from paraffin embedded tissue samples, one of which I am using currently to extract RNA from only formalin fixed tissue samples (while...

02 February 2018 8,221 2 View