Eric Liu

2 Questions 2 Answers 0 Followers

Questions related from Eric Liu

Are my HT1080 proliferation and migration assay seeding densities reasonable?

After infecting HT1080 cells with shScr, shCol18a1#3, and shCol18a1#4 viruses made from the pKLO.1 vector and selecting with puromycin, I will seed them in preparation for a proliferation and...

06 March 2023 3,010 2 View

How long does puromycin treatment usually take for adequate selection?

I am preparing for a proliferation assay where I have infected KP230 cells with shScr, shCol18a1#35, and shCol18a1#37 viruses. These shRNA viruses are made with the pLKO.1 vector and have...

22 July 2022 5,964 1 View