I had successful knockdown of eGFP in 293T cells two months ago and I can no longer get knockdown. What coud be the problem?

For my project, I need to use a plasmid that produces eGFP upon influenza infection. This is done by expressing RNAs containing antisense eGFP flanked by the 5' and 3' UTRs of the influenza NP,...

25 November 2013 9,355 8 View