Eric Dexter

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Questions related from Eric Dexter

Is there a compelling reason to avoid shearing DNA during extraction when the downstream application is short-read (100-300bp) Illumina sequencing?

Many DNA extraction protocols warn against vortexing during sample preparation as it tends to shear DNA into smaller fragments. This makes sense if the downstream application requires high...

20 December 2019 1,824 2 View

Running ANOVA on PCA principal component scores?

I'm reviewing a paper in which the authors conducted a PCA on some data that were a split among some a priori groups, and then looked for differences between those groups by running a separate...

06 February 2018 614 3 View

Expressing time as a circular variable

When considering time as a variable which undergoes cycles across a data-series (ex. Month of year across a multi-year time-series, or time of day in a multi-day study), it is often useful to...

15 February 2017 412 2 View

Does anyone have a good protocol for removing excessive sediment from zooplankton samples collected from turbid waters?

Samples were collected from a highly turbid river and contain much too much sediment for efficient taxonomic processing of individuals. Samples were filtered through a 75 micron mesh. Simply...

25 May 2016 8,234 5 View

Can anyone recommend alignment software to match short reads from one species to the reference genome of a related species?

I'm analyzing RADseq data from pooled samples of microcrustacean, and I need to use some sort of bioinformatics filter to remove all contaminating sequence data that belong to the stomach contents...

01 October 2015 2,739 5 View