Eric Raul Barros

2 Questions 4 Answers 0 Followers

Questions related from Eric Raul Barros

Can I use iBAQ values from MS/MS data and normalize them using another parameter (e.g. urinary creatinine)?

I performed MS/MS on urinary exosomes from two sets of patients, in order to determine differential expression. However, we found that intensity values vary greatly between samples, given that the...

13 May 2019 8,630 2 View

What housekeeping gene is the most appropriate when working with exosomes?

I am currently isolating exosomes from the apical and basolateral sides of polarized epithelial cells, in order to determine gene expression (mRNA/miRNA) through RT-PCR. However, when using U6, I...

01 January 1970 1,936 8 View