Eóin O'Brien

3 Questions 3 Answers 0 Followers

Questions related from Eóin O'Brien

Can anyone advise on the effectiveness of osmotic mini pumps (inserted subcutaneously) at increasing the serum concentration of a protein in mice?

We are currently working with NSG mice which are deficient in complement C5a. This protein is important for our disease model but due to it's short half-life repeated injections would be required...

29 September 2016 5,395 2 View

How would you test if a batch of TNF-alpha is biologically active?

I want to check if a batch of TNF-alpha is still biologically active and I am wondering if anyone knows the best way to do that? What readout after stimulation of cells with the TNF would give the...

30 July 2014 866 4 View

Can the THP-1 monocyte cell line be placed into the same subsets as primary monocytes i.e. classical, intermediate and non-classical monocytes?

Classical monocytes are characterised as CD14++ CD16-, Intermediate monocytes express CD14++CD16+ and Non-classical monocytes are characterised by CD14+CD16++ expression.

11 April 2014 7,525 2 View