Enrico König

3 Questions 16 Answers 0 Followers

Questions related from Enrico König

What else other than protein absorbs at 280nm in my sample when meassuring protein concentration at nanodrop?

Dear all, in our lab we are working on Outer Membrane Vesicles (OMVs). When we purify these vesicles we simply quantify the total protein content using nanodrop. Then we check the quality of the...

02 February 2018 9,365 11 View

How can I remove a low-copy plasmid from E. coli?

Dear all, in my lab we are working with E. coli. A couple of weeks ago we tried to remove a low-copy plasmid (pACYC) from one of our strains by simply growing it in the absence of the selective...

10 October 2017 4,695 2 View

Can anyone help me with a protocol to strip-off Ni from used Ni-NTA agarose for gravity-flow chromatography

Dear all, I am using Ni-NTA agarose (Qiagen) for purification of His-tagged proteins by gravity-flow chromatography. Given that this agarose is remarkably expensive I would like to re-use it. For...

03 March 2016 3,179 9 View