Enrico König

3 Questions 14 Answers 0 Followers

Questions related from Enrico König

What else other than protein absorbs at 280nm in my sample when meassuring protein concentration at nanodrop?

Dear all, in our lab we are working on Outer Membrane Vesicles (OMVs). When we purify these vesicles we simply quantify the total protein content using nanodrop. Then we check the quality of the...

27 February 2018 5,148 10 View

Who used the tryptophan operon as promotor?

Does anyone have experience using a tryptophan operon as promotor for the inhibition of heterologous expression in E. coli? If so, where can I get the sequence from? And what quantities of...

20 February 2018 7,043 0 View

How can I remove a low-copy plasmid from E. coli?

Dear all, in my lab we are working with E. coli. A couple of weeks ago we tried to remove a low-copy plasmid (pACYC) from one of our strains by simply growing it in the absence of the selective...

18 October 2017 9,709 2 View