3 Questions 4 Answers 0 Followers
Questions related from Emmanuele Crespan
Hi everyone, I need to calculate which fraction (percent) of a given oligo is annealed at given temperature (like 4°C, 10°C, 20 °C and 37°C ). I found the formula to calculate the Tm (50%...
12 December 2017 1,060 3 View
Hi everyone, I have a couple of high GC content oligonucleotides that I need to anneal each other. One of those is 6-Fam labeled. For me it is important to have all the 6-Fam primer annealed....
11 November 2017 7,372 2 View
As I understood, among AML patients, about 20% express terminal deoxynucleotidyl transferase (TdT). However I was not able to find a a AML cell line expressing TdT. Could you help me? I'll really...
02 February 2016 5,392 1 View
