Can anyone explain why my EGFP expression increased (but Ai9 RFP decreased -like normal) after imaging with a confocal microscope?

I have been trying to image a receptor in the brain for weeks now. It is an EGFP-cre G-coupled protein receptor. The model is a iCre based EGFP KI mouse model crossed with an Ai9 reporter line. I...

24 May 2024 7,721 2 View