Emma Niba

2 Questions 10 Answers 0 Followers

Questions related from Emma Niba

Why do I obtain high molecular weight bands/ smear as PCR product ?

Hello, I have been performing PCR with the same type of template, primer sets and enzyme at the same PCR condition and it was ok. Recently, I started observing high molecular weight bands / smear...

08 August 2018 8,312 23 View

Why does my cell line shows protein expression on western blotting and immunostaining, although no mRNA transcript is observed by PCR?

I am performing splicing of dystrophin in cancer cells. In one cell line, no conventional dystrophin transcript was successfully amplified by PCR. We supposed that this cell line habor multi...

09 September 2016 1,817 7 View