Emma Aneheim

2 Questions 5 Answers 0 Followers

Questions related from Emma Aneheim

Why won't my HiTrap desalting column perform as well as its NAP-5/10 counterparts in purifying radiolabeled antibodies?

Same media in both columns (GE Sephadex 25). Pre-treated with BSA in the same way. Flowrates within limits stated for he HiTrap. Still bad perfomance. The outcome is a pure product but a lot of...

01 January 2015 6,771 9 View

Any advice on contamination during gel filtration of Herceptin?

I'm using commercial Herceptin for some experiments and when running FPLC (column superdex 200, PBS mobile phase, eluting 0.5ml/min) on my samples I find, besides the antibody peak (UV 280nm) at...

11 November 2013 1,755 4 View