How long do I need to treat cells with Brefeldin A to prevent exocytosis bursts from the Golgi? Is there a better way to obtain this effect?

I want to acutely prevent budding of vesicles from the Golgi. Does anyone know of a good way to do this? I know that Brefeldin a works after 1 hour, but I want to know whether I can treat them for...

07 September 2014 3,977 4 View