Emma Niba

4 Questions 9 Answers 0 Followers

Questions related from Emma Niba

Why does my cell line shows protein expression on western blotting and immunostaining, although no mRNA transcript is observed by PCR?

I am performing splicing of dystrophin in cancer cells. In one cell line, no conventional dystrophin transcript was successfully amplified by PCR. We supposed that this cell line habor multi...

08 September 2016 5,947 7 View

What are the methods of checking gDNA contamination of cDNA?

I am analyzing alternative splicing of the dytsrophin gene in cancer cells and I have observed intron retention of many dystrophin introns in a particular cell line. I confirmed all intron...

23 October 2015 581 8 View

How can we estimate the age of a mutation?

Hi, I identified a mutation that is very frequent and could represent a founder effect in Vietnam. I would like to know the time that has elapsed since the appearance of the common ancestor...

14 July 2014 6,767 4 View

Mitochondria isolation by magnetic-activated cell sorting (MACS)?

My mitochondria isolated by MACS is always contaminated with cells because I can always detect GAPDH from the cDNA prepared from the supposed pure mitochondria. How do I get rid of this...

18 November 2013 4,970 12 View