Emma Leaseburg

2 Questions 2 Answers 0 Followers

Questions related from Emma Leaseburg

Are these bands too smeared for good qPCR results?

Below is a picture of a 1% gel of my qPCR products from salt marsh sediments (I know, its not the best picture). Standard and NTC are also labeled. I am wondering if my bands are too blurry to be...

13 October 2023 2,770 0 View

Why is the amplification of my target sequence occurring at an unusually low annealing temperature?

I am attempting to amplify nirS and nirK in some salt marsh sediment samples for my Masters thesis studying denitrification. My nirS gene is amplifying great in both PCR and qPCR. In the...

27 September 2023 6,858 3 View