Emin Bursa

2 Questions 2 Answers 0 Followers

Questions related from Emin Bursa

Almost 2x DNA conc. difference between Nanodrop & Qubit, is that normal?

I have been aware of why a Qubit should give more accurate and often lower DNA concentrations over the nanodrop. Today I finally did a direct comparison of the two (Nanodrop one, Qubit 4) and the...

29 September 2022 1,091 2 View

Getting low e.coli miniprep yields, how can I improve?

I'm trying to purify plasmids from e.coli, the plasmids are high copy. I start with about 1.5ml culture volume, using neb monarch miniprep kit, and rarely get concentrations higher than 20 ng/ul,...

14 December 2021 9,137 7 View