Is it necessary to fix frozen sections before safranin O/fast green/hematoxylin staining, or would they just need to be rehyrdrated?

I am using this protocol (https://www.scientistsolutions.com/forum/cytology-histology-and-ihc-organelle-and-cytoskeletal-staining/cartilage-staining) to do cartilage staining on organoids that...

12 December 2019 9,392 5 View