Does PMA/ionomycin stimulation effect Foxp3 detection by flow cytometry in mouse splenocytes?

I'm staining mouse splenocytes for Foxp3 using the eBioscience kit. I'm also staining the same cells (different tubes) for IL-17 and IFNg so I do a PMA/ionomycin/Brefeldin A stimulation on all the...

04 May 2015 8,293 5 View