Emily Toscano Guerra

3 Questions 9 Answers 0 Followers

Questions related from Emily Toscano Guerra

How is it possible to detect that my UPL probe is expired or does not work?

Hello everybody. I have performed efficiency tests for primers of 12 genes with UPL probes in a Lightcycler 480 to obtain the curves with 4 dilutions of cDNA. I obtained optimal results for 10...

21 January 2020 2,603 1 View

Which is the best method for Protein quantification: Nanodrop 280A or Bradford?

I have problem quantifying my protein. Actually, I am working with Magnetic nanoparticles (Dynabeads) to bind my RpsA protein with a Binding buffer (ph 8), with differents conditions of...

02 May 2016 9,230 17 View

Have you ever had problems with FBS bacterial contamination from a new unoponed bottle?

2 weeks ago I started an experiment with siRNAs to inhibit P21 expresion. The protocol needs the use of medium without Antibiotics. So, first I grew my cells (LnCap) in medium RPMI complete with...

01 January 1970 5,466 8 View