Emily Toscano Guerra

4 Questions 12 Answers 0 Followers

Questions related from Emily Toscano Guerra

How is it possible to detect that my UPL probe is expired or does not work?

Hello everybody. I have performed efficiency tests for primers of 12 genes with UPL probes in a Lightcycler 480 to obtain the curves with 4 dilutions of cDNA. I obtained optimal results for 10...

21 January 2020 2,730 1 View

Which is the best method for Protein quantification: Nanodrop 280A or Bradford?

I have problem quantifying my protein. Actually, I am working with Magnetic nanoparticles (Dynabeads) to bind my RpsA protein with a Binding buffer (ph 8), with differents conditions of...

02 May 2016 9,291 17 View

What is the minimum amount of flavonoids that a person should consume daily?

There are a lot of publications about the estimated Dietary Flavonoid Intake, but this information is variable according to location (country) and socioeconomic group. However, none mentions what...

04 March 2014 5,688 11 View

Have you ever had problems with FBS bacterial contamination from a new unoponed bottle?

2 weeks ago I started an experiment with siRNAs to inhibit P21 expresion. The protocol needs the use of medium without Antibiotics. So, first I grew my cells (LnCap) in medium RPMI complete with...

01 January 1970 5,522 8 View