Emilia Stec-Martyna

2 Questions 3 Answers 0 Followers

Questions related from Emilia Stec-Martyna

What is the best protocol of histones isolation from bone marrow?

I am planning to analyze post-translational modifications, including acetylation and methylation of human histones, H3 and H4, via mass spectrometry (LC-ESI-Q-ToF). Can I ask for some advice and...

06 June 2014 1,238 2 View

How big can I expect the peptides (what m/z range) to be when using ESI-LC-MS analysis after trypsin digestion?

Generally, I obtain something between 700-2800 Da. How can I get smaller fragments? Is it just the type of enzyme we use?

03 March 2014 4,743 3 View